**70**wrote:

When plotting flowFrames, I sometimes get the warning, "Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'.

I have a general sense of what this is telling me in terms of the density plot, but I cannot figure out how to set the gridsize from the high order plotting function. Can anyone help point me to the right documentation or a specific syntax / work-around?

fs1 = read.flowSet(path = path, pattern = '.fcs')

> plot(fs1[[1]])

There were 50 or more warnings (use warnings() to see the first 50)

> warnings()

Warning messages:

1: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

2: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

3: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

4: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

5: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

6: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

7: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

8: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth) :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

9: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

10: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

11: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

12: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

13: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

14: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

15: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

16: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

17: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

18: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

19: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

20: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

21: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

22: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

23: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

24: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

25: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

26: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

27: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

28: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

29: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

30: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

31: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

32: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

33: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

34: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

35: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

36: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

37: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

38: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

39: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

40: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

41: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

42: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

43: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

44: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

45: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

46: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

47: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

48: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

49: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

50: In KernSmooth::bkde2D(x, gridsize = nbin, bandwidth = bandwidth, ... :

Binning grid too coarse for current (small) bandwidth: consider increasing 'gridsize'

>

**1.2k**• written 2.9 years ago by Robert Baer •

**70**