I thank you about your guidances. But i still have problematic through import BigWig file or any Project as project = 'SRP009615'.
Despite that i check the version of "rtracelayer" that is (1.34.2 for bioc-release), furthermore i have been tried to apply all commands which is (bioc-devel mailing list "https://stat.ethz.ch/pipermail/bioc-devel/2016-May/009336.html, ")
like the following
|1- x <- import.bw('http://duffel.rail.bio/recount/DRP000366/bw/DRR000897.bw',|
+ as = 'RleList'
2- url <- unshorten_url('http://duffel.rail.bio/recount/DRP000366/bw/DRR000897.bw')
4-bw <- BigWigFile(url)
5- x <- import.bw(url, as = 'RleList') ; x <- import.bw(bw, as = 'RleList')
+ destfile = 'DRR000897.bw', method = 'curl', extra = '-L')
HERE it told me i don't have 'curl'
that processed the same my case but until this moment i couldn't to fix that problem which said me
[[[2017-02-28 16:59:34 loadCoverage: loading BigWig file http://duffel.rail.bio/recount/SRP009615/bw/mean_SRP009615.bw
Too many pushAbortHandlers, can only handle 11
Error in seqinfo(con) : UCSC library operation failed]]]
i will be very thankful to guide me what is the optimal solution concerning this problem.