Error message for installing DESeq2
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mdroog ▴ 10
@mdroog-13422
Last seen 6.0 years ago

Yesterday I managed to install DESeq2 on my home computer but today it simply won't work on my computer at work.

I install it using the following command:

source("https://bioconductor.org/biocLite.R")
biocLite("DESeq2")

 

However, when I then use the command

library(DESeq2)

I get the following:

> library(DESeq2)
Loading required package: GenomicRanges
Loading required package: GenomeInfoDb
Error: package or namespace load failed for ‘GenomeInfoDb’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]):
 there is no package called ‘GenomeInfoDbData’
Error: package ‘GenomeInfoDb’ could not be loaded

 

And so I installed GenomicRanges and GenomeInfoDb using biocLite, but I keep getting this error message.

 

What should I change?

 

> sessionInfo()
R version 3.4.1 (2017-06-30)
Platform: x86_64-w64-mingw32/x64 (64-bit)
Running under: Windows >= 8 x64 (build 9200)

Matrix products: default

locale:
[1] LC_COLLATE=English_United States.1252  LC_CTYPE=English_United States.1252   
[3] LC_MONETARY=English_United States.1252 LC_NUMERIC=C                          
[5] LC_TIME=English_United States.1252    

attached base packages:
[1] parallel  stats4    stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] Biobase_2.36.2       edgeR_3.18.1         limma_3.32.2         BiocInstaller_1.26.0 IRanges_2.10.2      
[6] S4Vectors_0.14.3     BiocGenerics_0.22.0 

loaded via a namespace (and not attached):
[1] compiler_3.4.1  tools_3.4.1     RCurl_1.95-4.8  grid_3.4.1      locfit_1.5-9.1  bitops_1.0-6    lattice_0.20-35
 

>

deseq2 • 6.7k views
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@james-w-macdonald-5106
Last seen 2 days ago
United States

Did you try installing GenomeInfoDbData using biocLite?

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I did but then I get the following messages:

> source("https://bioconductor.org/biocLite.R")
Bioconductor version 3.5 (BiocInstaller 1.26.0), ?biocLite for help
> biocLite("GenomeInfoDb")
BioC_mirror: https://bioconductor.org
Using Bioconductor 3.5 (BiocInstaller 1.26.0), R 3.4.1 (2017-06-30).
Installing package(s) ‘GenomeInfoDb’
also installing the dependency ‘GenomeInfoDbData’

trying URL 'https://bioconductor.org/packages/3.5/bioc/bin/windows/contrib/3.4/GenomeInfoDb_1.12.2.zip'
Content type 'application/zip' length 7073643 bytes (6.7 MB)
downloaded 6.7 MB

package ‘GenomeInfoDb’ successfully unpacked and MD5 sums checked

The downloaded binary packages are in
	C:\Users\m.droog\AppData\Local\Temp\Rtmp4Yo5FY\downloaded_packages
installing the source package ‘GenomeInfoDbData’

trying URL 'https://bioconductor.org/packages/3.5/data/annotation/src/contrib/GenomeInfoDbData_0.99.0.tar.gz'
Content type 'application/x-gzip' length 16238792 bytes (15.5 MB)
downloaded 15.5 MB

'\\srv-data1\home$\M87E6~1.DRO\R\R-34~1.1' is not recognized as an internal or external command,
operable program or batch file.

The downloaded source packages are in
	‘C:\Users\m.droog\AppData\Local\Temp\Rtmp4Yo5FY\downloaded_packages’
Old packages: 'Formula', 'scater'
Update all/some/none? [a/s/n]: 
a

  There are binary versions available but the source versions are later:
        binary source needs_compilation
Formula  1.2-1  1.2-2             FALSE
scater  1.3.35  1.4.0              TRUE

  Binaries will be installed
trying URL 'https://bioconductor.org/packages/3.5/bioc/bin/windows/contrib/3.4/scater_1.3.35.zip'
Content type 'application/zip' length 7883976 bytes (7.5 MB)
downloaded 7.5 MB

package ‘scater’ successfully unpacked and MD5 sums checked

The downloaded binary packages are in
	C:\Users\m.droog\AppData\Local\Temp\Rtmp4Yo5FY\downloaded_packages
installing the source package ‘Formula’

trying URL 'https://cran.rstudio.com/src/contrib/Formula_1.2-2.tar.gz'
Content type 'application/x-gzip' length 150946 bytes (147 KB)
downloaded 147 KB

'\\srv-data1\home$\M87E6~1.DRO\R\R-34~1.1' is not recognized as an internal or external command,
operable program or batch file.

The downloaded source packages are in
	‘C:\Users\m.droog\AppData\Local\Temp\Rtmp4Yo5FY\downloaded_packages’
Warning messages:
1: running command '"//srv-data1/home$/M87E6~1.DRO/R/R-34~1.1/bin/x64/R" CMD INSTALL -l "\\srv-data1\home$\m.droog\R\R-3.4.1\library" C:\Users\M87E6~1.DRO\AppData\Local\Temp\Rtmp4Yo5FY/downloaded_packages/GenomeInfoDbData_0.99.0.tar.gz' had status 1 
2: In install.packages(pkgs = doing, lib = lib, ...) :
  installation of package ‘GenomeInfoDbData’ had non-zero exit status
3: running command '"//srv-data1/home$/M87E6~1.DRO/R/R-34~1.1/bin/x64/R" CMD INSTALL -l "\\srv-data1\home$\m.droog\R\R-3.4.1\library" C:\Users\M87E6~1.DRO\AppData\Local\Temp\Rtmp4Yo5FY/downloaded_packages/Formula_1.2-2.tar.gz' had status 1 
4: In install.packages(update[instlib == l, "Package"], l, repos = repos,  :
  installation of package ‘Formula’ had non-zero exit status
> library(GenomeInfoDb)
Error: package or namespace load failed for ‘GenomeInfoDb’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]):
 there is no package called ‘GenomeInfoDbData’
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R does not like the 'network' file path "\\srv-data1\home$\m.droog..."; google for 'map network drive' to associate this path with a letter, so that the path is "z:\...".

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Yes, thank you. That was it indeed. Instead of the network drive, R is now running on the local D:\\ disk. This solved the problem.

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Hi!!

I am having this same problem you had. How you solved it?

Thanks in advance!!!

Ida

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Did you not see the comment you replied to (where the OP said it was fixed) and the previous comment (where Martin told the OP how to fix it)?

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I did and I got this other error:

> biocLite(c("GenomeInfoDb"))
BioC_mirror: https://bioconductor.org
Using Bioconductor 3.7 (BiocInstaller 1.30.0), R 3.5.1 (2018-07-02).
Installing package(s) ‘GenomeInfoDb’
also installing the dependency ‘GenomeInfoDbData’

trying URL 'https://bioconductor.org/packages/3.7/bioc/bin/windows/contrib/3.5/GenomeInfoDb_1.16.0.zip'
Content type 'application/zip' length 3830739 bytes (3.7 MB)
downloaded 3.7 MB

package ‘GenomeInfoDb’ successfully unpacked and MD5 sums checked

The downloaded binary packages are in
	C:\Users\Ida.G.PantojaFelicia\AppData\Local\Temp\RtmpOyhiyI\downloaded_packages
installing the source package ‘GenomeInfoDbData’

trying URL 'https://bioconductor.org/packages/3.7/data/annotation/src/contrib/GenomeInfoDbData_1.1.0.tar.gz'
Content type 'application/x-gzip' length 8204728 bytes (7.8 MB)
downloaded 7.8 MB

'\\natia7ndenas2\DPS\IDAG~1.PAN\DOCUME~1\R\R-35~1.1' is not recognized as an internal or external command,
operable program or batch file.
In R CMD INSTALL

The downloaded source packages are in
	‘C:\Users\Ida.G.PantojaFelicia\AppData\Local\Temp\RtmpOyhiyI\downloaded_packages’
Old packages: 'R6', 'rstudioapi', 'stringi'
Update all/some/none? [a/s/n]: 
a

  There are binary versions available but the source versions are later:
           binary source needs_compilation
R6          2.2.2  2.3.0             FALSE
rstudioapi    0.7    0.8             FALSE
stringi     1.1.7  1.2.4              TRUE

  Binaries will be installed
trying URL 'https://cran.rstudio.com/bin/windows/contrib/3.5/stringi_1.1.7.zip'
Content type 'application/zip' length 14368013 bytes (13.7 MB)
downloaded 13.7 MB

package ‘stringi’ successfully unpacked and MD5 sums checked

The downloaded binary packages are in
	C:\Users\Ida.G.PantojaFelicia\AppData\Local\Temp\RtmpOyhiyI\downloaded_packages
installing the source packages ‘R6’, ‘rstudioapi’

trying URL 'https://cran.rstudio.com/src/contrib/R6_2.3.0.tar.gz'
Content type 'application/x-gzip' length 30968 bytes (30 KB)
downloaded 30 KB

trying URL 'https://cran.rstudio.com/src/contrib/rstudioapi_0.8.tar.gz'
Content type 'application/x-gzip' length 47394 bytes (46 KB)
downloaded 46 KB

'\\natia7ndenas2\DPS\IDAG~1.PAN\DOCUME~1\R\R-35~1.1' is not recognized as an internal or external command,
operable program or batch file.
In R CMD INSTALL
'\\natia7ndenas2\DPS\IDAG~1.PAN\DOCUME~1\R\R-35~1.1' is not recognized as an internal or external command,
operable program or batch file.
In R CMD INSTALL

The downloaded source packages are in
	‘C:\Users\Ida.G.PantojaFelicia\AppData\Local\Temp\RtmpOyhiyI\downloaded_packages’
Warning messages:
1: In install.packages(pkgs = doing, lib = lib, ...) :
  installation of package ‘GenomeInfoDbData’ had non-zero exit status
2: In install.packages(update[instlib == l, "Package"], l, repos = repos,  :
  installation of package ‘R6’ had non-zero exit status
3: In install.packages(update[instlib == l, "Package"], l, repos = repos,  :
  installation of package ‘rstudioapi’ had non-zero exit status
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Exactly. And what did Martin say you should do in order to fix that?

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Look, I did the 'map network drive' and I am still getting that error I just posted.

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Evidently it didn't work, because R is still trying to install to the network drive rather than the mapping you say you set up.

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The troublesome looking line in your output is

'\\natia7ndenas2\DPS\IDAG~1.PAN\DOCUME~1\R\R-35~1.1' 

What does R.home() report? It should have the mapped network drive, with a form like W:\\...

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Hi Martin,

Thanks so much for your response.

This is what I get when I do R.home():

> R.home()
[1] "//natia7ndenas2/DPS/IDAG~1.PAN/DOCUME~1/R/R-35~1.1"
 

I am in a Government Computer and I am not sure if I am having some restrictions. When I did the google search for map network drive and followed the steps, I selected the letter but in the Folder Section, Browse, I only see one option for Network. I can't select any other Folder because is empty. Otherwise, I typed the path in the space provided for "Folder". It created a mapped network drive because I see it under My PC but when I run again the command, does not work. It is not connecting the letter. That's why I think there are some restrictions and I have to contact IT people. But I could be wrong and there is some step I am not doing.

Thanks again!

Ida

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It is not unreasonable to ask your local help desk to support you in this.

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