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Comment: How to reverse complement some elements of a DNAStringSet
by
Debbie Sutcliffe
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To reverse complement specific sequences in a DNAStringSet, utilize lapply combined with an ifelse function. Instead of subsetting, apply t…
Comment: Differential expression of FPKM from RNA-seq data using limma and voom()
by
Essam
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0
Gordon I appreciate your useful response here. Since the original fastq files were created before the GEO era, we are unable to locate them…
Comment: How to reverse complement some elements of a DNAStringSet
by
Christine Jones
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0
Fantastic, thank you
Comment: When running contrasts, does it use normalized read counts?
by
Katherine
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0
Ok, so the comment I was given where they said DeSeq2 should be with normalized counts is incorrect.
the ```normalized_counts``` variable …
Answer: How to reverse complement some elements of a DNAStringSet
by
James W. MacDonald
67k
You should always try the vectorized method first, rather than looping.
```
> myFasta[myFasta_rc] <- reverseComplement(myFasta[myFasta_…
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