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Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance by Michael Love 41k

With 3 vs 3, when one sample increases from 50 to 5000, this can lead to rejection of null hypothesis. I'm not surprised with the above cou…

Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance by samer • 0

This is the result i got when i only looked on day 04 for the gene in example 1 posted above dds_sub <- dds[, dds$date %in% c("day04"…

Answer: What is the meaning of "CpG interrogation" and "single nucleotide extension(SBE) by James W. MacDonald 64k

There are two types of probes used by Illumina. The type I probes bind directly to the CpG site and have two different probes (one for a me…

Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance by Michael Love 41k

Then for the remaining genes that you don't think are DE, just plot like this: ``` dds_sub <- dds[,dds$condition %in% c("x","y")] dropleve…

Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance by samer • 0

yes i turned it off @mikelove

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