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Comment: Deseq2, enrichGO and ensembl ID
by
James W. MacDonald
67k
I am not sure I understand. You say that a unique Ensembl ID maps to multiple gene symbols. That doesn't mean you have two genes (it's just…
Answer: Why did PCA plots look exactly the same after adjusting for batch covariate in D
by
James W. MacDonald
67k
All you have done is set up a `DESeqDataSet` object and then run `vst` on it. That won't affect the PCA plot at all - you are simply tellin…
Comment: Newbie Question - adding metadata to an analysis
by
Bryan H
• 0
Makes sense now, thanks for the help!
Comment: Deseq2, enrichGO and ensembl ID
by
Quang
• 0
Hi James -- I hope to piggy back on this question of how to deal with the ensemble ID with version ID suffix. In contrast to https://suppor…
Comment: Newbie Question - adding metadata to an analysis
by
James W. MacDonald
67k
You don't need to generate an `ExpressionSet`, as you already have a `GeneFeatureSet`, which extends `eSet` and is a better fit for what yo…
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A: Trajectory analysis for single cell sequencing
Answer: Annotation with org.Hs.eg.db Genome Build 38
Answer: Annotation with org.Hs.eg.db Genome Build 38
Answer: Considerations behind the choice of RNAseq Differential Expression Analysis Tool
A: goana limma- extract list of DE genes and genes in the enriched GO terms?
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