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Wondering how to look at the reads of a gene in samples to check if it was knocked out?
knockout
DESeq2
written 18 months ago by
Lillian
• 0
1 result • Page
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Comment: EuroBioC2023 abstract submission closing soon!
by
Jeremy
• 0
Thanks for the reminder! If you're a Bioconductor user or developer, make sure to [tabs][1] submit your abstract by the deadline, Monday, A…
Comment: Mean variance curve troubleshoot filtering
by
gingergeiger22
• 0
Gordon, thank for your response. 'filterByExpr()' was marginally better. I can see high variability in samples within the groups from the M…
Comment: What is the importance of `--countReadPairs` in featureCounts on paired end data
by
rohitsatyam102
▴ 20
Since I don't know which organism you are working on, I would recommend using the following: `-s 0` if library is unstranded `-C` Discard…
Comment: What is the importance of `--countReadPairs` in featureCounts on paired end data
by
rohitsatyam102
▴ 20
Yes use this option. It is recommended for PE and if you don't use it you will observe nearly twice the number of counts in your results. H…
Comment: Incorporating Salmon offset matrix into Wilcoxon DEG analysis
by
Andre
• 0
I edited the previous post. I meant to remove the estimateGLMRobustDisp line in the second snipper : ```` DGE_list_object <- estimateGLMR…
Votes
Comment: Comparing different method for DESEQ2
Answer: How do I fix an odd voom plot in a combined dataset?
Answer: How do I fix an odd voom plot in a combined dataset?
Comment: How do I fix an odd voom plot in a combined dataset?
C: How does edgeR cpm function calculate log(CPM) values?
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