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Comment: In WGCNA which is the matrix on which the eigen decomposition is run to get the
by
andres.firrincieli
▴
50
cross-posted on r/bioinformatic:[link][1]
To calculate the module eigengenes with `moduleEigengenes()` will need the normalized expression…
Comment: How to normalize long-read RNA-seq data for comparison with short-reads
by
Jonathan Göke
▴
30
You could have a look at the bambu package which is designed for long read /nanopore RNA-Seq and which handles the normalisation issue for …
Comment: AnnotationForge::makeOrgPackage "argument is missing, with no default" Errors
by
liuchangbio
•
0
I think I know the reason for the error, it was caused by an extra comma in the last line, and after deleting it, the problem was solved!
Y…
Comment: Possible bugs in Rsubread/stad-alone featureCounts options fracOverlap and large
by
Wei Shi
★
3.5k
Thank you for reporting the bug and apologies for late response. We are currently working on fixing this bug. A fixed version of Rsubread s…
Comment: Error in .order_seqlevels(chrom_sizes[, "chrom"]) : !anyNA(m32) is not TRUE
by
vs2001
▴
10
Getting the same error again. My code worked fine last night but it fails at this line today:
seqlevelsStyle(annotations) <- 'UCSC'
>…
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