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Answer: Normalisation using clinical traits in RNA seq using DESeq2
by
ATpoint
★
4.2k
See the vignette, it's a FAQ: https://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#why-after-vst-are-there-st…
Comment: More regions in union when increasing DBA$config$mergeOverlap
by
Ian D.
▴
70
Please could you comment on this @rory
Comment: Using featureCounts with SAF annotation file gives "Error: only GTF files contai
by
Wei Shi
★
3.6k
Your bam file names should be stored in a vector and then provided to the `files` parameter.
Comment: Volcano plot of scRNA-seq data not "centered"
by
Michael Love
42k
Agree with @atpoint
Depending on the "groups" in single cell, the question of DE can be not well defined without inserting additional inf…
Answer: Volcano plot of scRNA-seq data not "centered"
by
ATpoint
★
4.2k
Sounds like a normalization problem, but I am not sure how to help with that. Indeed you can use control genes, for example use the top 20%…
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