**10**wrote:

Hi,

I am using the qvalue R package to calculate qvalues of out of this pvalues distribution:

The lowest qs are around 0.22.

If we have a look at the p-values histogram, we see that there are actually more low ps than expected by chance. But with a q cut-off at 0.15 there is only one marker resulting as significant. When raising the threshold to 0.25 instead, the number of significant tests raise to 10'000.

I think this is due to the fact that low ps frequency decreases toward 0, therefore making it not possible to calculate respective qvalues.

How do you interpret this? Is there a way to obtain a qvalue in this situation?

thank you

Os

**130**• written 4 months ago by oliver.selmoni •

**10**