dear ALL how to find DE genes using both of 51bp data and 100bp data i have 12 samples, 6 of which are 100bp long, others are 51bp long 100bp datas cluding two conditions: treated1 treated2, treated3 vs. control1,control2,control3 50bp data can be seen as technical replicates of 100bp data?cluding two conditions: treated1 treated2, treated3 vs. control1,control2,control3 do u know how to analyze them? do i need classify them into 4 groups? or 2 groups? -- shan gao Room 231(Dr.Fei lab) Boyce Thompson Institute Cornell University Tower Road, Ithaca, NY 14853-1801 Office phone: 1-607-254-1267(day) Official email:sg839 at cornell.edu Facebook:http://www.facebook.com/profile.php?id=100001986532253