I just want to confirm WB data about expression of a particular gene. I see GEO has uploaded RNA-seq data sets. All I want to do is see expression levels of GSDMD, GSDME in MEF/BMDM cells. This isnt DEGs analysis I just want to see basal expression in WT for these 2 genes. All the guides I see are for comparing two populations. I dont know how to analyze raw counts or RPKM. What is the easiest way to do this?
For example, GSE165500 has RNAseq data for 3 UNX_BMDM replicates (both raw counts and RPKM). How do I take these files and generate a simple expression graph of 1 gene in 1 condition?