I'm working through the flowCore manual "flowCore: data structures package for flow cytometry data, N. Le Meur F. Hahne B. Ellis P. Haaland, December 1, 2016"
In section 4.3 Subsetting it instructs to give the code
> morphGate <- norm2Filter("FSC-H", "SSC-H", filterId="MorphologyGate", scale=2) > smaller <- Subset(fs, morphGate) # fs is a flowSet > fs[]
When I input my flowSet, it doesn 't work unless I specify a specific flowFrame,
The error I get:
> morphGate <- norm2Filter("FSC-A","SSC-A", filterId = "MorphologyGate", scale= 2) > smaller <- Subset(set,morphGate) # set is a non-transformed flowSet # Error in y[, 1] : incorrect number of dimensions
Up until this point, the flow set has been running fine with the previous code with the same flowSet.