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Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
by
Gordon Smyth
53k
Normalization or filtering are never done for subsets of samples in any limma or edgeR analysis. These things must always be done globally.
Answer: DESeq2 interactions
by
swbarnes2
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1.4k
The simpler way to do the first four comparisons is to make a new column in colData that is temp_pollution, and specify that column alone a…
Comment: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
by
James W. MacDonald
68k
You are. The reason I pointed you to `forgeBSgenomeDataPkgFromTwobitFile` is because it's much simpler, doesn't require a seed file, and is…
Comment: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
by
mat149
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I've tried the following, but I must be missing something:
setwd("C:\\Users\\....\\wd\\dump\\seqs_srcdir")
e122<-readDNA…
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