Bioconductor KeepSeqlevels

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GenomeInfoDb keepSeqlevels

updated 8.0 years ago by Valerie Obenchain ★ 6.8k • written 8.0 years ago by Chao-Jen Wong ▴ 40

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Answer: Consistent Decimals In Data After Antibody Array Background Correction Using Lim by Gordon Smyth 51k

`nec` computes one set of negative control parameters (mu, sigma, alpha) for each sample.

Comment: DESeq Input from Salmon + tximport/tximeta by LuciaNhu • 0

Thank you to the author for the quick reply. If I use `gene_counts_length_scaled.tsv` as a metric that has already normalized for library s…

Answer: DESeq Input from Salmon + tximport/tximeta by Michael Love 42k

Given nf-core's pipeline here, you can use rounded counts from `gene_counts_length_scaled.tsv`.

Comment: Question about using LRT test for time course experiments with DESeq2 by James W. MacDonald 67k

You don't want to add a logFC on top like that, as it invalidates the p-value. Maybe I wasn't clear before. The LRT gives you all the gene…

Comment: Question about using LRT test for time course experiments with DESeq2 by Denise • 0

Hi James, Thank you very much for your very detailed explanation! I guess my question is simpler than that, I just want to union, across…

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Answer: DESeq Input from Salmon + tximport/tximeta

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