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Comment: Average biological replicates in RNA-seq
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boczniak767
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740
@atpoint Should I first standardise data and then average replicates?
Comment: 3-factor DESeq2 trime-series analysis
by
gogeni5529
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Thans you @swbarnes2 for the answer.
I can see why trying to include all three parameters might be difficult to interpret. But we're inte…
Comment: 3-factor DESeq2 trime-series analysis
by
swbarnes2
★
1.4k
What does your PCA look like? A lot of times, I find that different cell lines are so different from each other, that they should not be d…
Answer: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
yongjie.wang
•
0
Thanks for your reply.
" normFactors <- exp(-1 * offst(set2))
normFactors <- normFactors / exp(rowMeans(log(normFactors)))
normalizat…
Answer: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I'm not familiar with this usage, or code here
```
normFactors <- exp(-1 * offst(set2))
```
In the RNA-seq gene-level workflow, we …
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