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single cells
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Some questions for remove batch effect by using mnnCorrect
batch effect
single cells
mnnCorrect
scran
updated 3.9 years ago by
Aaron Lun
★ 28k • written 3.9 years ago by
xingxd16
▴ 20
0
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619
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Can DropletUtils Package be used to identify cells for 10X VDJ data ?
DropletUtils
10X 5` TCR
single cells
updated 4.0 years ago by
Aaron Lun
★ 28k • written 4.0 years ago by
xingxd16
▴ 20
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Answer: Not sure what an Asymmetric MA plot means?
by
ATpoint
★ 2.4k
You asked this here already https://www.biostars.org/p/9558379/ with advise to do more prefiltering and checking normalization with control…
Comment: RUVr batch correction doubling number of DEGs
by
Zainab
• 0
Thanks Michael! This has really helped me put together my analysis!
Comment: Does it make sense to have so many statistically significant gene differentially
by
Michael Love
39k
Use group in ggplot to connect the pairs.
Answer: DispEsts plot
by
Michael Love
39k
You have a lot of high dispersion genes, check PCA plots, also count plots for the genes with high mean and high dispersion.
Answer: Correct script for featurecounts in Rsubread
by
Gordon Smyth
47k
See - https://bioconductor.org/packages/release/workflows/vignettes/RnaSeqGeneEdgeRQL/inst/doc/edgeRQL.html#quantifying-read-counts-for-eac…
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Comment: RUVr batch correction doubling number of DEGs
Comment: RUVr batch correction doubling number of DEGs
Answer: RUVr batch correction doubling number of DEGs
A: LogFC: how do you determine the cutoff for differentially expressed genes?
Confused about design for RNA seq experiment
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