Bioconductor Transcription factor

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transcription factor tf activation gene regulatory network gene expression

updated 8.0 years ago by caroline.green.c91 • 0 • written 8.1 years ago by Diwan • 0

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Comment: What is the importance of `--countReadPairs` in featureCounts on paired end data by rohitsatyam102 ▴ 20

Since I don't know which organism you are working on, I would recommend using the following: `-s 0` if library is unstranded `-C` Discard…

Comment: What is the importance of `--countReadPairs` in featureCounts on paired end data by rohitsatyam102 ▴ 20

Yes use this option. It is recommended for PE and if you don't use it you will observe nearly twice the number of counts in your results. H…

Comment: Incorporating Salmon offset matrix into Wilcoxon DEG analysis by Andre • 0

I edited the previous post. I meant to remove the estimateGLMRobustDisp line in the second snipper : ```` DGE_list_object <- estimateGLMR…

Comment: Incorporating Salmon offset matrix into Wilcoxon DEG analysis by Gordon Smyth 52k

Sorry, I had forgotten estimateGLMDisp() produces weights as well as NB dispersions. The weights from estimatGLMRobustDisp() were intended …

Answer: Mean variance curve troubleshoot filtering by Gordon Smyth 52k

You could use `filterByExpr()`, as recommended by Law et al (2018) and in the limma and edgeR case studies. However, the main problem wi…

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Comment: Comparing different method for DESEQ2

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