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josmantorres
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Comment: Error in .order_seqlevels(chrom_sizes[, "chrom"]) : !anyNA(m32) is not TRUE
by
Lucia
•
0
I am getting the same error from yesterday. Does somebody know what it is going on? or How can we fix it?
Or is it a matter of time to get…
Answer: batch effect correction before DESeq2 analysis
by
ATpoint
★
2.1k
This is covered in the vignette. Including batch into the design is recommending for DE testing and removal from transformed counts is reco…
Answer: Reproducibility using limma for DE Analysis
by
TSauer
•
0
Yes, i know that. However, I implemented the imputation step quite long ago, using a different package, exported the data and then did the …
Comment: Reproducibility using limma for DE Analysis
by
Gordon Smyth
47k
limma can also be run without imputation, i.e., leaving the missing values as NA.
Comment: Reproducibility using limma for DE Analysis
by
TSauer
•
0
Hey Gordon,
thank you for the swift response.
Indeed, I do perform imputation. Weirdly, it did not occur to me that this could be the origi…
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