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Comment: Deseq2 and Normalization of RNA sequencing data
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Is this quesion related to your earlier question? https://support.bioconductor.org/p/9157127/
If you've already done an edgeR analysis, yo…
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Your first method would be valid if, instead of taking ratios, you instead used the differences between the log-transformed 13C and 12C int…
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You have discovered something that I have pointed out many times on this forum. fgsea does not implement the classic GSEA method published …
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In that case both time and treatment are identical things, so choose one and go with it.
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