Bioconductor HPC

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855

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JobPosting AI HPC CuratedAtlasQueryR

19 months ago Stefano Mangiola ▴ 40

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3.3k

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Regression limma STATISITICS HPC

updated 21 months ago by Gordon Smyth 53k • written 21 months ago by Jitendra ▴ 10

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868

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featureCount Rsubread HPC

2.3 years ago kthapa • 0

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4.3k

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HPC R Bioconducter

3.8 years ago Jitendra ▴ 10

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955

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HPC profiling performance

4.2 years ago Jack • 0

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2.0k

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Minfi local installation Centos hpc

updated 7.7 years ago by Kasper Daniel Hansen ★ 6.5k • written 7.7 years ago by Goku • 0

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Comment: Clarification on counting in Rsubread (featureCounts) by Gordon Smyth 53k

Have you told featureCounts to do strand-specific counting? As pointed out by Frances Turner, you can lose reads if they overlap the gene …

Answer: Clarification on counting in Rsubread (featureCounts) by Frances Turner ▴ 10

You should look at the summary files produced by feature counts. This gives a breakdown of what happened to reads that were not assigned. Y…

Comment: Incosistencies in buildSNNGraph() function by ATpoint ★ 5.0k

Just played with this a bit and I think it comes down to not having a fixed seed before running the leiden thingy. Same goes for PCA and UM…

Answer: Incosistencies in buildSNNGraph() function by Aaron Lun ★ 29k

The most obvious thing that comes to mind is the RNG seed. `cluster_leiden()` does some random number generation, somewhere deep in the **i…

Comment: Opportunity to host EuroBioC2026 Preconference workshops! by TikTsokaas33ocissalpc • 0

Thank you for sharing the EuroBioC2026 workshop opportunity. I m interested in hosting a hands on session teaching reproducible data analys…

Votes

C: ControlGenes/ housekeeping genes Deseq2

Answer: Clarification on counting in Rsubread (featureCounts)

C: RNA-Seq analysis with Limma/edgeR, time course experiment, aberrant results

A: Conversion from counts to FPKM

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Great Question to fl ▴ 20

Popular Question to Lluís Revilla Sancho ▴ 760