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How to make the data compatible for DESeq2?
DESeq2
TPM
rawcounts
StringTie
updated 2.2 years ago by
Michael Love
42k • written 2.2 years ago by
snijesh
▴ 200
0
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595
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Is it possible to do batch correction on Ht-Seq raw counts instead of using normalized counts? What's the difference?
Normalisedcounts
Rawcounts
Ht-SeqRNAData
BatchEffect
2.7 years ago
Ety
• 0
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Comment: Volcano plot has many values around zero
by
nramakr
• 0
Thank you. Could you clarify what that says about the validity of the whole dataset? Would it still be alright to assess candidates above t…
Answer: Volcano plot has many values around zero
by
ATpoint
★ 4.5k
It means you have little (no) evidence for any differential expression.
Comment: Differential Expression Analysis with unbalanced batches without replication bet
by
Thili
• 0
Thank you for your reply. I currently have batch-corrected data processed with scvi-tools (specifically, totalvi) and have followed the sam…
Comment: Problem at champ.load
by
andrew.gibbons
• 0
I'd love to know if anyone has a workaround too. I've tried rolling back ChAMP and R to the previous versions that others have said work wi…
Comment: GOstats kegg analysis in wheat - help - none of the objects in the first column
by
GV Yoshikawa
• 0
Thank you for the reply. I tried swapping columns before asking, it made no difference. head(keggframeData) path_id kegg…
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Answer: Combining RNAseq and microarray data using edgeR/limma-voom
Answer: How do I merge a list of GRanges?
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