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Ascona Workshop “Biological systems: from first principles to data-driven modelling and back” 27. 3. - 1. 4. 2022
theory
modeling
statistics
ascona
Workshop
2.6 years ago • updated 15 months ago
Wolfgang Huber
★ 13k
2
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2
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DSS understanding delta option and posterior probability
posterior pobability
statistical test
theory
updated 4.0 years ago by
James W. MacDonald
65k • written 4.0 years ago by
stephanie.rialle
• 0
11
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5
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7.4k
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Theoretical WGCNA Question
WGCNA
Theory
Scale-Free Topology
updated 26 days ago by
Garrett
• 0 • written 9.1 years ago by
brohawndg
▴ 150
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Comment: How to approach time course RNAseq data? Seeing 80% DE genes in timepoint groups
by
Gordon Smyth
50k
The NG dispersion is almost zero for your data, meaning that there is almost no biological variation between the replicates. There are also…
Comment: Can I trust the differential expression analysis when the two groups overlap on
by
BioinfGuru
▴ 30
No worries, wish I could be more help. Plotly provide a [3-D plot][1] function that's pretty simple (the two you did are still 2-D plots, t…
Comment: Can I trust the differential expression analysis when the two groups overlap on
by
Michael Love
42k
If you manually compute PCA on VST data, what are the top 5 genes contributing to PC1? https://github.com/thelovelab/DESeq2/blob/devel/R/p…
Comment: Can I trust the differential expression analysis when the two groups overlap on
by
Eric Katagirya
• 0
I created a DESeqDataSet object using DESeqDataSetFromMatrix function. I don't anticipate any mismatching or mixup of sampleIDs.
Answer: Assymetric MA plot with no overall trend
by
Michael Love
42k
> so many upregulated genes vs. downregulated, which I think is worrying Take a look at the plotCounts for a couple of the up-regulated …
Votes
Answer: Can log2cpm be used for proteomics data normalization?
Ensembl 112 has been released.
A: Diffbind results - non uniform width
A: Different logFC (log2foldchange) values for genes from limma-voom and other too
Answer: Error EPICv2 ChAMP
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