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Gordon Smyth
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Comment: Best practice for quantifying sncRNA
by
Abhishek
•
0
Thanks, I will try out Bowtie.
Is GeneGlobe and Qiagen the same company ?
https://www.qiagen.com/us/resources/download.aspx?id=bea2dcfa-…
Answer: Using both spike in and TMM normalizations in ChIP-seq samples
by
Gordon Smyth
43k
It is impossible to use both spike-in or TMM normalization. You must do one or the other. They replace each other, they cannot be combined.…
Comment: Best practice for quantifying sncRNA
by
ATpoint
▴
700
If the sequences are 20-something bp long then it appears logical to me to throw away any reads that are longer than that after adapter tri…
Comment: P value and Adj. P value showing N/A
by
ATpoint
▴
700
http://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html#why-are-some-p-values-set-to-na
As Mike says, you can be…
Comment: Using both spike in and TMM normalizations in ChIP-seq samples
by
ATpoint
▴
700
I just gave my two cents on spike-ins and normalization diagnostic over at biostars (see link below) which you may want to have a look at. …
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