Bioconductor Deep sequencing

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isoform deep sequencing voom

updated 3.1 years ago by Gordon Smyth 48k • written 3.1 years ago by Ina Hoeschele ▴ 620

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deep sequencing fasta bam trim

6.0 years ago achaillon • 0

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Comment: DataFrame Construction With Non-standard Column by Hervé Pagès 16k

SimpleList is a List derivative and List derivatives in general don't need protection. For example, a GRangesList object (which is also a L…

Answer: Deseq2, how to model design for comparing Mutant mouse at age 14 days versus Mu by swbarnes2 ★ 1.3k

The Status grouping you show above is good to use if you want to simply compare one subset of samples to another subset. The ~Genotype +…

Answer: Remove duplicates of colData in TreeSummarizedExperiment by Michael Love 40k

Copying my code from Slack for posterity. James' code is more streamlined for this step I think. ``` # make a dataset that has duplicate s…

Comment: DataFrame Construction With Non-standard Column by James W. MacDonald 63k

Oh, ok. I understand. Technically both should work according to what the help page for `DataFrame` says (both `SimpleList` and `list` have …

Comment: DeSeq2 design fails (full model matrix is less than full rank) by Basti ▴ 720

As recommended, you can only perform ~group. You can use contrasts do perform the comparison AA5 vs FHIN1 (see [DESeq2 vignette][1]), but t…

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A: collapseReplicates in DESeq2

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