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Answer: After running samtools, the featureCounts command crashed.
by
Kevin Blighe
★
4.0k
Hello Rui Tang,
The buffer overflow error in featureCounts that you encountered is likely due to a compatibility issue between the Subre…
Answer: Problem with the TMB plot
by
Kevin Blighe
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4.0k
The issue that you describe with the tumor mutational burden (TMB) plots appearing linear and lacking consistent TMB values across samples …
Answer: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
by
Kevin Blighe
★
4.0k
There are alternative explanations for why these samples are so similar. The similarity could result from low biological variability in gen…
Comment: Post translational modifications and phosphoproteomics in limpa?
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james.eapen
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0
Thank you!
Comment: Highly similar RNA-seq samples in PCA - pooling or technical duplication?
by
ATpoint
★
5.0k
The donor effect is crystal clear, and absent in these suspicious samples. It strongly argues for a technical problem. If you really need s…
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