Bioconductor Gene specific efficiencies

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ddCt efficiency gene specific efficiencies

updated 5.9 years ago by Zhang, Jitao David ▴ 120 • written 5.9 years ago by bettenbrock • 0

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Comment: Joining different datasets and analyzing using the group-specific condition effe by James W. MacDonald 68k

There is an example of how you can do this in the [DESeq vignette][1]. An alternative would be to fit a linear mixed model using the `vari…

Comment: Joining different datasets and analyzing using the group-specific condition effe by jason.chai • 0

@mikelove Could I please ask if a similar method would work my set of samples? I have 255 samples which have been generated from 76 subject…

Comment: Clarification on counting in Rsubread (featureCounts) by Luca • 0

Thanks to both of you for your helpful suggestions. The counting was already strand-specific at both the exon and gene levels. I resolved …

Comment: Clarification on counting in Rsubread (featureCounts) by Gordon Smyth 53k

Have you told featureCounts to do strand-specific counting? As pointed out by Frances Turner, you can lose reads if they overlap the gene …

Answer: Clarification on counting in Rsubread (featureCounts) by Frances Turner ▴ 20

You should look at the summary files produced by feature counts. This gives a breakdown of what happened to reads that were not assigned. Y…

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C: ControlGenes/ housekeeping genes Deseq2

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