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glmgampoi
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Recommendations for DESeq2 Independent Filtering with Pseudobulk Data
DESeq2
independentfiltering
glmGamPoi
13 months ago • updated 12 months ago
Bob
▴ 30
1
vote
8
replies
1.7k
views
Advice on appropriateness of an offset in glmGamPoi/edgeR
DESeq2
glmGamPoi
offset
edgeR
scRNAseq
updated 14 months ago by
Gordon Smyth
53k • written 14 months ago by
Alec
• 0
1
vote
3
replies
945
views
Dispersion estimation step is very slow with latest DESeq2 and glmGamPoi
DESeq2
glmGamPoi
18 months ago
I-Hsuan Lin
▴ 10
8
votes
7
replies
2.9k
views
Scaling DEXSeq estimateDispersions for large datasets, glmGamPoi?
DESeq2
glmGamPoi
DEXSeq
updated 2.2 years ago by
alexandresegers
▴ 20 • written 2.9 years ago by
jeremymsimon
▴ 20
1
vote
1
reply
2.5k
views
Instaling glmGamPoi
glmGamPoi
updated 2.8 years ago by
ATpoint
★ 4.9k • written 2.8 years ago by
sina.tadayon
• 0
0
votes
3
replies
1.8k
views
Can't install packages due to '503 Service Unavailable'
glmGamPoi
BiocManager
updated 2.8 years ago by
Vincent J. Carey, Jr.
6.7k • written 2.8 years ago by
i.am.filippov
• 0
5
votes
4
replies
3.2k
views
Using DESeq2 with GlmGamPoi
DESeq2
glmGamPoi
scRNAseq
4.5 years ago
tatyana.perlova
• 0
7 results • Page
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Comment: Heatmap of the Count Matrix
by
ArnulfoByrd
• 0
Perhaps PCA loadings offer a more comprehensive perspective. Now, thinking back, I recall struggling with a similar data reduction dilemma …
Answer: readBam vs read_bam_insert_metrics
by
James W. MacDonald
68k
Depends on what you mean by input. If you mean to ask if you use `readBam` to generate something to use as an argument to `read_bam_insert_…
Answer: How to construct a BSgenome object from a custom genome assembly using BSgenomeF
by
James W. MacDonald
68k
There is an example in the help page for `forgeBSgenomePkgFromTwobitFile`. Have you tried emulating that?
Answer: Guidance on limma for pseudobulk scRNA-seq with multi-factor design
by
Gordon Smyth
53k
What you describe sounds a good approach to me. I would also use sample weights (`sample.weights=TRUE` when running `voomLmFit`) and robust…
Comment: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I moved your post from an "answer" to a "comment". I don't know about `offst()` function, what is the scale or direction, or whether you a…
Votes
Answer: Limpa Pipeline Order
A: How barcode-plot enrichment is calculated?
A: How barcode-plot enrichment is calculated?
Answer: ROAST compare results with complex design
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
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