updated 5.0 years ago by
Robert Castelo
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written 5.0 years ago by
rubi
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Comment: Best practice for quantifying sncRNA
by
Abhishek
•
0
k has to be odd - trying 9, 21 instead
Comment: Best practice for quantifying sncRNA
by
Abhishek
•
0
I did use the genome as decoy.
And I used whole transcriptome from Gencode for indexing. Maybe I should index using just non coding transc…
Comment: Best practice for quantifying sncRNA
by
Abhishek
•
0
Thank you for your suggestions.
Throwing away larger reads sounds true, I'll try using Cutadapt for that besides adapter trimming.
I did …
Comment: Best practice for quantifying sncRNA
by
Abhishek
•
0
Thanks, I will try out Bowtie.
Is GeneGlobe and Qiagen the same company ?
https://www.qiagen.com/us/resources/download.aspx?id=bea2dcfa-…
Answer: Using both spike in and TMM normalizations in ChIP-seq samples
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Gordon Smyth
43k
It is impossible to use both spike-in or TMM normalization. You must do one or the other. They replace each other, they cannot be combined.…
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