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updated 3.9 years ago by Michael Love 42k • written 3.9 years ago by bbj23 • 0

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5.7 years ago sbbinfo90 • 0

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Comment: How to add Ensembl ids after Pseudobulk analysis by DESeq2 by BioinfGuru ▴ 30

I would use biomaRt. This is what happens when annotation occurs to early. If possible I would run the DEG analysis on the original output…

Comment: Volcano plot of scRNA-seq data not "centered" by ATpoint ★ 4.2k

My comment was generic. If your experiment causes drastic shifts then the situation might be different. The advise is the same: Try to use …

Comment: conumee with Infinium MethylationEPIC v2.0 by Maximus • 0

Hi Sole, If there is a combined mixture of MSet of 450K, EPIC and EPICv2, how should I create an annotation object? I tried to create anno…

Comment: Volcano plot of scRNA-seq data not "centered" by tjl • 0

Thank you both. The code is below, simplified a bit for simplicity. ATpoint, your suggestion re: the top 20% of genes by baseMean makes me …

Comment: Cannot install oligo on Fedora by James W. MacDonald 66k

Wait a minute. The `oligo` package is *supposed to* be using `zlibbioc`, but apparently it is not. I get the same thing as you do, where it…

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A: Analyzing data without replicates using DiffBind

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Popular Question to Robert Castelo ★ 3.3k