Feature Counts -R XS Read Assignment Tag
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@b9ada64c
Last seen 4 months ago
Australia

When running FeatureCounts with -R (report reads) the output bam file reads are tagged with XS, XN and XT.

I have bam files aligned with HISAT2 which writes the XS tag to store positive and negative strand (+,-).

Therefore each read line in the output bam file contains 2 XS tags and subsequent downstream software such as SAMTOOLS view -d tag filtering fails to correctly filter by XS tag. Is it possible to modify the FeatureCounts XS tag to another tag ID?

Thanks in advance, Chris

FeatureCounts Rsubread • 348 views
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