updated 2.6 years ago by
Konstantinos Yeles
▴
90
•
written 2.7 years ago by
Sooni
▴
10
1
vote
1
reply
936
views
Recent ...
Replies
Answer: After running samtools, the featureCounts command crashed.
by
Kevin Blighe
★
4.0k
Hi Rui Tang,
The "buffer overflow detected" error in featureCounts usually stems from a corrupted or malformed GTF file, often due to tr…
Answer: Problem with the TMB plot
by
Kevin Blighe
★
4.0k
Hi,
The linear TMB plot with no clustering suggests your variant calls may lack sample-specific discrimination, likely due to filtering ap…
Answer: gene Set with high AUC score but no cells assigned to cell type
by
Kevin Blighe
★
4.0k
Hi,
You have correctly identified the issue: AUCell's automatic threshold selection is conservative by design, aiming for specificity to…
Answer: featureCounts -L: high unassigned:mapq fraction
by
Kevin Blighe
★
4.0k
Hi Katarzyna,
The discrepancy arises because your samtools stats were restricted to genic regions only, where alignments are typically o…
Answer: Streamlining the computing time for MiloDE p-value correction in large dataset?
by
Kevin Blighe
★
4.0k
Hi Joseph,
I agree with ATpoint's suggestion that pseudobulk aggregation (e.g., via aggregateData in scran or similar) would likely be far…
Awards
• All
Scholar to
James W. MacDonald
68k
Popular Question to
Daniel Cameron
▴
40
Popular Question to
Peter Hickey
▴
760
Locations
• All
Barcelona, Spain, 30 minutes ago
Chile, 43 minutes ago
United Kingdom, 2 hours ago
The Cave, 181 Longwood Avenue, Boston, USA, 3 hours ago
United States, 3 hours ago
Barcelona, Spain, 30 minutes ago
Chile, 43 minutes ago
United Kingdom, 2 hours ago
The Cave, 181 Longwood Avenue, Boston, USA, 3 hours ago
United States, 3 hours ago
Traffic: 544 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6