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Martin Morgan
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written 6.2 years ago by
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Answer: After running samtools, the featureCounts command crashed.
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Kevin Blighe
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Hi Rui Tang,
The "buffer overflow detected" error in featureCounts usually stems from a corrupted or malformed GTF file, often due to tr…
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Kevin Blighe
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Hi,
The linear TMB plot with no clustering suggests your variant calls may lack sample-specific discrimination, likely due to filtering ap…
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Kevin Blighe
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Hi,
You have correctly identified the issue: AUCell's automatic threshold selection is conservative by design, aiming for specificity to…
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Kevin Blighe
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Hi Katarzyna,
The discrepancy arises because your samtools stats were restricted to genic regions only, where alignments are typically o…
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Hi Joseph,
I agree with ATpoint's suggestion that pseudobulk aggregation (e.g., via aggregateData in scran or similar) would likely be far…
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