Bioconductor Ht-seq

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RNA-seq featureCounts ht-seq

written 3.4 years ago by ag1805x ▴ 80

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Answer: Using STAR's readspergene.tab.out outputs to make gene-level count matrix for DE by James W. MacDonald 64k

The short answer is that you don't. `tximport` is meant to summarize transcript abundance estimates at the gene level, and as you might ima…

Comment: Inconsistent results in DiffBind by ATpoint ★ 3.6k

One is the "raw" logFCs, not corrected for the standard error of the logFCs, the other one the shrunken logFCs from DESeq2 lfcShrink, "corr…

Answer: Can't download Bioconductor by James W. MacDonald 64k

[You need to update][1]. [1]: https://cran.r-project.org/web/packages/BiocManager/vignettes/BiocManager.html#basic-use

Comment: Can't download Bioconductor by ATpoint ★ 3.6k

Please see especially the 1st and 2nd point in https://www.bioconductor.org/help/support/posting-guide/#keypoints

Comment: Small RNA-seq Analysis DESEQ2 : Unexpected Significance by Michael Love 41k

With 3 vs 3, when one sample increases from 50 to 5000, this can lead to rejection of null hypothesis. I'm not surprised with the above cou…

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Answer: WGCNA documentation not available

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