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openprimer
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9
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1.5k
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How to load fasta file with openPrimeR?
openPrimeR
written 2.8 years ago by
marongiu.luigi
• 0
0
votes
4
replies
428
views
Load templates from openPrimeR
PCRmultiplex
openPrimeR
10 months ago • updated 9 months ago
Santiago Edilberto
• 0
1
vote
3
replies
935
views
How to define settings for design primers with openPrimeR?
openPrimeR
2.8 years ago
marongiu.luigi
• 0
0
votes
2
replies
601
views
How to save primers in local file in openPrimeR
openPrimeR
updated 9 months ago by
Santiago Edilberto
• 0 • written 2.8 years ago by
marongiu.luigi
• 0
0
votes
1
reply
495
views
How to increase the number of primer set output in openPrimeR
openPrimeR
updated 2.4 years ago by
matthias-doering
▴ 40 • written 2.8 years ago by
marongiu.luigi
• 0
0
votes
1
reply
500
views
openPrimer check_constraints cannot compute Tm
openPrimeR
updated 18 months ago by
matthias-doering
▴ 40 • written 19 months ago by
wnussbau
• 0
0
votes
1
reply
230
views
How to save primers in a local file with openPrimeR
openPrimeR
updated 8 months ago by
Basti
▴ 740 • written 9 months ago by
Santiago Edilberto
• 0
0
votes
1
reply
501
views
openPrimeR does not recognize external MELTING installation
openPrimeR
updated 2.4 years ago by
matthias-doering
▴ 40 • written 2.4 years ago by
benedikt.kirchner
• 0
0
votes
0
replies
556
views
openPrimeR can't find external dependencies
openPrimeRui
openPrimeR
4.5 years ago
Vinicius Henrique da Silva
▴ 40
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Comment: Correct the batch effect in methylation analysis in R
by
Gordon Smyth
49k
> Is it okay to apply removeBatchEffect before a limma analysis(limma linear model)? No, you should not apply any batch correction before …
Comment: Correct the batch effect in methylation analysis in R
by
kyj2226
• 0
Thank you for your response Gordon :) I've already run the code below, just add batch to the limma linear model design <- model.matri…
Answer: Correct the batch effect in methylation analysis in R
by
Gordon Smyth
49k
You should not apply batch correction functions like harman before a limma analysis. Instead, just add `batch` to the limma linear model. …
Answer: Agilent Microarray Gene Oncology analysis gone wrong
by
Gordon Smyth
49k
Your use of `coef=1` is incorrect, for reasons explained in the limma User's Guide. If you had simply left `coef` at the default, then thin…
Comment: Normalised counts from RUVg in RUVSeq
by
Shaimaa Gamal
• 0
Hello I was wondering if I should use ruv-corrected counts and ruv lib sizes to create a DGE object that can be used later for DE analysis …
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Answer: Correct the batch effect in methylation analysis in R
Answer: enrichGOgradient color change
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Answer: Filter genes by expression for voomLmFit
Answer: Filter genes by expression for voomLmFit
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