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shrna
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Using edgeR for small shRNA-seq screen analysis
edger
shrna
8.5 years ago
sirintra
▴ 10
2
votes
2
replies
2.3k
views
Analysis of shRNA screen data
shrna
edger
deseq2
differential expression
normalized counts
9.1 years ago
Jane Merlevede
▴ 90
1
vote
1
reply
1.2k
views
limma for shRNA dropout screen analysis?
limma
gmap
shRNA
dropout screen
updated 9.3 years ago by
Aaron Lun
★ 29k • written 9.3 years ago by
Anna Dvorkin
▴ 40
4
votes
4
replies
2.0k
views
edgeR:processAmplicons barcode match only 57% when it should be 100% -- I artificially inserted them -- are there other assumptions
edgeR
processamplicons
shrna
updated 9.8 years ago by
thomas.leete
• 0 • written 9.9 years ago by
Anne Deslattes Mays
▴ 10
0
votes
5
replies
3.1k
views
example fastq file, sampleAnnotaition and Hairpin file for shRNAseq/EdgeR tool
shRNAseq
edgeR
shRNA
updated 10.0 years ago by
Gordon Smyth
53k • written 10.0 years ago by
KB
▴ 50
5 results • Page
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Answer: Can I set the normalizationFactors of DEseq2 object as 1 after using RUVg?
by
Michael Love
43k
I'm not familiar with this usage, or code here ``` normFactors <- exp(-1 * offst(set2)) ``` In the RNA-seq gene-level workflow, we …
Answer: Which of apeglm and ashr may be more appropriate for pseudobulked DESeq2 analysi
by
Michael Love
43k
> apeglm appears much more aggressive in shrinkage, pushing many genes toward logFC = 0 > ashr maintains a gradient of shrinkage val…
Comment: voomLmFit fitted log-CPM values
by
Gordon Smyth
53k
The fitted values are on the log2CPM scale. I would have told you if they were not! I think you may be getting confused by the differences…
Comment: voomLmFit fitted log-CPM values
by
Lina
▴ 10
Is the output from fitted(v) in the log2 CPM scale? or CPM scale?
Answer: voomLmFit fitted log-CPM values
by
Gordon Smyth
53k
Fitted values are obtained from limma MArrayLM objects by ``` fitted(v) ```
Votes
A: How barcode-plot enrichment is calculated?
A: How barcode-plot enrichment is calculated?
Answer: ROAST compare results with complex design
How to account for solvent controls in DESeq2 when comparing follicular vs luteal phase?
Answer: How to account for solvent controls in DESeq2 when comparing follicular vs lutea
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