Bioconductor SinglecellRNA-Seq

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singlecellRNA-Seq pathview statistics R

3.7 years ago • updated 3.6 years ago Luo Weijun ★ 1.6k

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R statistics pathview singlecellRNA-Seq

4.0 years ago • updated 3.7 years ago Luo Weijun ★ 1.6k

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Answer: RSubread output significantly different from bash Subread output by Gordon Smyth 52k

The difference (as ATpoint has correctly pointed out) is that Rsubread::featureCounts counts multi-mapping reads by default whereas the com…

Answer: Deseq2 Error in solve.default(qr.R(qrx)) : 'a' (1 x 0) must be square by Michael Love 43k

I would check the rank of your model matrix, and perhaps meet with a local statistician who can help you create an appropriate design matri…

Comment: sva: get a sv for RIN scores by gabriel.hoffman ▴ 170

Agreed. I'll add that with small sample size, a covariate can explain some fraction of expression variation even under the null.

Comment: RSubread output significantly different from bash Subread output by Aimee • 0

Thank you! I will try rerunning with that parameter and see if it fixes the discrepancy! I'll report back. Update: This completely resolve…

Answer: RSubread output significantly different from bash Subread output by ATpoint ★ 4.6k

The R version counts multimappers (countMultiMappingReads is TRUE by default) while the command line version does not. It's beyond me why t…

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