Bioconductor Spike-ins

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DiffBind Chipseq spike-ins

updated 4.0 years ago by Rory Stark ★ 5.2k • written 5.0 years ago by Konstantin Okonechnikov ▴ 40

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diffbind chip-seq chip spike spike-ins

updated 4.4 years ago by Rory Stark ★ 5.2k • written 4.5 years ago by Theo ▴ 10

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Comment: Question about using LRT test for time course experiments with DESeq2 by Denise • 0

Not really, we are testing some toxic compounds to identify genes that are activated in response to the toxic exposure. Genes that change o…

Comment: Combining RNAseq and microarray data using edgeR/limma-voom by Adam Marstrand • 0

Hi again, Thank you for the response! Can you expound on why it is not a good idea? I realize that the expression levels/differential expr…

Answer: Combining RNAseq and microarray data using edgeR/limma-voom by James W. MacDonald 67k

No, that's not a good idea at all. You should be doing a meta-analysis with these data rather than trying to combine like that. `GeneMeta` …

Answer: GOstats kegg analysis in wheat - help - none of the objects in the first column by James W. MacDonald 67k

Check the help page for `KEGGFrame`. Your columns are out of order.

Comment: Question about using LRT test for time course experiments with DESeq2 by James W. MacDonald 67k

You are testing the interaction, so you are asking for those genes that change at any time *differently, depending on the treatment*. If co…

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Answer: DecideTest vs topTAGs in edgeR

Comment: DESeq Input from Salmon + tximport/tximeta

Answer: DESeq Input from Salmon + tximport/tximeta

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