Entering edit mode
Nilofer Ali
•
0
@f4d593ce
Last seen 3.4 years ago
I am new to DESEQ2 and RNA quantification and analysis in R. I have been given a dataset of 60k rows (of genes) and16 columns for 4 treatment conditions comprising of 4 repeat cell sample each. I am just confused to import the data into R and hoe to normalise the biological replicates to perform differential analysis of gene expression between these conditions? ```
What tutorials have you read?
This guidance to DESeq2 http://bioconductor.org/packages/devel/bioc/vignettes/DESeq2/inst/doc/DESeq2.html and RNA-seq workflow: gene-level exploratory analysis and differential expression
The vignette opens with many many pages about of all the different ways you can import data into DESeq. Which ones have you tried?