SeSAMe DMR for EPIC V2
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luca.s ▴ 50
@lucas-24386
Last seen 1 day ago
Italy

Hi, we are analyzing EPIC V2 arrays with SeSAMe v.1.23.2. When we follow the github tutorial and try and identify DMR, with the DMR function specifying platform="EPICv2", the following error pops up: Merging correlated CpGs ... Error in seq_len(n.cpg - 1) : argument must be coercible to non-negative integer Error during wrapup: 'length = 4' in coercion to 'logical(1)'

No error if we specify "EPIC" as platform. Does anyone have a hint on how to solve this? At the bottom you find the versions for the different packages used. Thanks

R: 4.4.0 Bioconductor: 3.20 sesame: 1.23.2 sesameData: 1.23.0 ExperimentHub: 2.13.0

Code should be placed in three backticks as shown below


# include your problematic code here with any corresponding output 
# please also include the results of running the following in an R session 

sessionInfo( )
sesame EPICv2manifest epicv2 • 139 views
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zhouwanding ▴ 20
@zhouwanding-15106
Last seen 2 days ago
United States

My guess is that the input has a suffix-removed probe id for EPICv2 data. We will add a fix to that in the DMR function in a few days. But can you verify this is the case?

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Update: The new 1.23.8 should have fixed this issue. Let me know if it still doesn't.

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Thank you, that was indeed the case. I fed the function with the data containing the replicate probes collapsed by mean and this caused the error.

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