Bioconductor Bioc2023

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presentation BioC2023 Conference community Bioconductor

updated 8 weeks ago by Ramon • 0 • written 20 months ago by Maria Doyle ▴ 90

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Comment: How to reverse complement some elements of a DNAStringSet by Christine Jones • 0

Fantastic, thank you

Comment: When running contrasts, does it use normalized read counts? by Katherine • 0

Ok, so the comment I was given where they said DeSeq2 should be with normalized counts is incorrect. the ```normalized_counts``` variable …

Answer: How to reverse complement some elements of a DNAStringSet by James W. MacDonald 67k

You should always try the vectorized method first, rather than looping. ``` > myFasta[myFasta_rc] <- reverseComplement(myFasta[myFasta_…

Answer: When running contrasts, does it use normalized read counts? by James W. MacDonald 67k

A contrast is a comparison of two or more coefficients. When you run `nbiomWaldTest` you are fitting the GLM and estimating coefficients. T…

Comment: Too much data for Diffbind by Ou, Jianhong ★ 1.3k

Just in case if somebody still want to apply summits greater than 0 and got the same error, you can filter out the peaks with start positio…

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Comment: featureCounts fails to load annotation file

Answer: KEGGgraph result difference

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