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DESeq2
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0
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4
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805
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Higher Dimensional RNASeq Clustering Significance
DESeq2
RNASeq
PrincipalComponent
pcaExplorer
written 18 months ago by
James
• 0
0
votes
4
replies
242
views
Volcano plot of scRNA-seq data not "centered"
DESeq2
updated 3 days ago by
ATpoint
★ 4.2k • written 5 days ago by
tjl
• 0
1
vote
8
replies
200
views
Slightly different count plots from R and spreadsheet
DESeq2
ggplot2
7 days ago • updated 6 days ago
boczniak767
▴ 720
0
votes
3
replies
142
views
GSVA function
DESeq2
GSVA
ssGSEA
updated 2 days ago by
Robert Castelo
★ 3.3k • written 5 days ago by
michelafrancesconi8
• 0
0
votes
2
replies
115
views
How to add Ensembl ids after Pseudobulk analysis by DESeq2
Seurat
DESeq2
single-cell
scRNA
Pseudobulk
updated 2 days ago by
Guido Hooiveld
★ 4.0k • written 3 days ago by
Sara
• 0
0
votes
2
replies
114
views
Normalisation using clinical traits in RNA seq using DESeq2
DESeq2
clinicaltraits
Normalization
batchnormalisation
5 days ago • updated 2 days ago
sukeshinik5
• 0
0
votes
5
replies
104
views
Counter-directional LFC shrinkage
DESeq2
1 day ago • updated 1 hour ago
ennui
• 0
0
votes
1
reply
40
views
LRT, pairwise comparisons and p-value adjustment
DESeq2
updated 1 hour ago by
James W. MacDonald
66k • written 23 hours ago by
boczniak767
▴ 720
8 results • Page
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Comment: Using featureCounts with SAF annotation file gives "Error: only GTF files contai
by
atan
• 0
Thank you! I ended up running it with a gtf on command line, partially because I couldn't figure out how to list multiple files in R for in…
Comment: Using featureCounts with SAF annotation file gives "Error: only GTF files contai
by
atan
• 0
For some reason, that's still not working for me on command line. I ended up figuring out another way around, though, by converting the g…
Answer: LRT, pairwise comparisons and p-value adjustment
by
James W. MacDonald
66k
The convention is to adjust only within comparisons. But as the analyst, that's your call.
Comment: Counter-directional LFC shrinkage
by
ennui
• 0
Thank you for your response. The `ashr` shrinkage indeed preserves more of the original ranks in the data. So if I understand correctly, L…
Comment: Counter-directional LFC shrinkage
by
ennui
• 0
I agree it looks offset (and weird). The data is from NGS, not RNAseq, but we still want to see relative abundance between conditions - I t…
Votes
Reduce the number of nodes appear in the cnetplot of clusterprofiler
A: DESeq2: add annotations (from data frame) to DESeqDataSet
Answer: Error in gseDO function
Comment: what the test method for enrichGO in clusterProfiler?
A: Comparison between two different group's pathway analysis results
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Guido Hooiveld
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