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deseq2 LRT RNA-Seq

updated 10.5 years ago by Michael Love 43k • written 10.5 years ago by solgakar@bi.technion.ac.il ▴ 90

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Answer: Help with defining groups by Gordon Smyth 53k

Defining sample groups is separate to reading in the DIA-NN data, because the sample conditions and covariates are not necessarily coded in…

Comment: Sharing a collection of datasets in ExperimentHub by Peter Hickey ▴ 760

The [**scRNAseq**](https://bioconductor.org/packages/scRNAseq/) package ([source on GitHub](https://github.com/LTLA/scRNAseq)) might serve …

Comment: Using DESeq2 on single cell RNA Seq data of same cell type but different conditi by ReinholdGaylord99 • 0

Thank you so much! https://www.nature.com/articles/s41467-021-25960-2/ [geometry dash lite](https://geometrydashlite.online)

Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design by Gordon Smyth 53k

I'm not sure that I understand. You have a dataset that, even after pseudo-bulking, still has 6000 samples? That is surprising. Retaining …

Comment: Guidance on limma for pseudobulk scRNA-seq with multi-factor design by abaed • 0

Hello again @gordonsmyth, I've now applied the analysis using a more detailed annotation of cell types/clusters, which includes about 100 …

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Comment: readDNAStringSet with non DNA characters

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