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PCR
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Analyze Amplicon Seq results for variants and mutation sites
virus
pcr
amplican
mutations
13 months ago
Sara
• 0
0
votes
1
reply
675
views
PCR simulation with PNAs
PCR
DECIPHER
PNA
4.5 years ago
marissaruthlee
• 0
0
votes
6
replies
3.0k
views
LIMMA on RT-PCR data
limma
pcr
updated 5.7 years ago by
Gordon Smyth
50k • written 10.4 years ago by
Sandhya Pemmasani Kiran
▴ 40
0
votes
1
reply
1.3k
views
Minimum number of genes to use R package "nondetects"
nondetects
R
PCR
updated 6.6 years ago by
Matthew McCall
▴ 830 • written 6.6 years ago by
garciaii_antuerpia
• 0
0
votes
1
reply
996
views
Normalization and batch effect adjustment of fluidgm pcr data to be analyzed with Limma
fluidigm
normalization
limma
pcr
updated 7.2 years ago by
Gordon Smyth
50k • written 7.2 years ago by
raf4
▴ 20
5 results • Page
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Comment: DEG Filtering
by
Gordon Smyth
50k
RMA normalization is good but batch correction using NOISeq isn't right. Data shouldn't be batch corrected before the limma analysis and NO…
Answer: maftools: Error in validateMaf(maf = maf, isTCGA = isTCGA, rdup = removeDuplicat
by
snijesh
▴ 20
You can try: using `read.delim` or `fread`. ``` my_maf = data.table::fread("path/to/maf/sample.maf") my_maf = read.maf(maf = my_maf) …
Comment: DEG Filtering
by
Amit
• 0
Hi Gordon, Thank you for your response and advice. I want to analyze Dataset GSE18090 (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi) whic…
Comment: edgeR, 3 x 2 design with a batch effect and contrasts
by
Gordon Smyth
50k
Yes, that is a quote from [A guide to creating design matrices for gene expression experiments](https://bioconductor.org/packages/release/w…
Comment: edgeR, 3 x 2 design with a batch effect and contrasts
by
JKim
• 0
Thank you. I think I found why the order matters. > Although an intercept-free design matrix has been coded using the 0+ notation, the in…
Votes
Answer: edgeR, 3 x 2 design with a batch effect and contrasts
Answer: padj-based gradient scale from barplot (clusterProfiler results) does not work p
C: How to check that the reads in two fastq files are in the same order
Answer: Need help with creating and plotting temporal clustering analysis of DEGs
Answer: Problem converting msigdb Human symbols to Zebrafish in gmt format
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