Biostar Deep sequencing

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isoform deep sequencing voom

updated 2.5 years ago by Gordon Smyth 47k • written 2.5 years ago by Ina Hoeschele ▴ 620

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deep sequencing fasta bam trim

5.3 years ago achaillon • 0

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Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper by Gordon Smyth 47k

Am I understanding correctly that you have 24 RNA-seq libraries in total, 6 from the pooled samples and 18 from regular samples with biolog…

Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper by k.roelfs • 0

I'm sorry, I know it is kinda confusing... We have 6 pools in total: Phenotype A: T1a, T2, T1b, T1c Phenotype B: T1, T2 Each pool consist…

Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper by Gordon Smyth 47k

Sorry, I don't understand your experiment. You are reading the edgeR Section on on what to do without replicates, but your experiment appe…

Answer: MA plot by Nathalie • 0

Thank you very much for your help and comments. There is errors in my file. BaseMean is BaseMeanLog2. I don't know how insert a file.

Comment: Design for DE analysis in tidybulk by Michael Love 39k

The mapping from what you want to a contrast is a long conversation. You can make pairwise comparisons or numeric contrasts or really anyth…

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Volcano Plot in R

Answer: MA plot

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