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Answer: Not sure what an Asymmetric MA plot means?
by
ATpoint
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2.4k
You asked this here already https://www.biostars.org/p/9558379/ with advise to do more prefiltering and checking normalization with control…
Comment: RUVr batch correction doubling number of DEGs
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Zainab
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Thanks Michael! This has really helped me put together my analysis!
Comment: Does it make sense to have so many statistically significant gene differentially
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Michael Love
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Use group in ggplot to connect the pairs.
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Michael Love
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You have a lot of high dispersion genes, check PCA plots, also count plots for the genes with high mean and high dispersion.
Answer: Correct script for featurecounts in Rsubread
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Gordon Smyth
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See
- https://bioconductor.org/packages/release/workflows/vignettes/RnaSeqGeneEdgeRQL/inst/doc/edgeRQL.html#quantifying-read-counts-for-eac…
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