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ERCC
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1.1k
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Incorporating RUVg results into ImpulseDE2 model
ImpulseDE2
ImpulseDE
ERCC
RUVSeq
3.6 years ago • updated 3.5 years ago
Mallory
▴ 10
3
votes
2
replies
3.8k
views
Using the ERCC spike as a normalization factor for a custom algorithm
ERCC
rna-seq
edgeR
tmm normalised values
updated 8.2 years ago by
Aaron Lun
★ 28k • written 8.2 years ago by
what_theheck24
• 0
27
votes
7
replies
16k
views
Usability of ERCC spike-ins in standard RNAseq experiments
RNAseq
deseq2
ercc
updated 8.6 years ago by
Simon Anders
★ 3.8k • written 8.6 years ago by
mbeckste
▴ 30
16
votes
5
replies
8.3k
views
DESeq and Limma+Voom Normalization for Rna-Seq Data Using Ercc Spike-In
limma
voom
deseq
normalization
ercc
updated 9.5 years ago by
Gordon Smyth
52k • written 9.5 years ago by
komal.rathi
▴ 120
8
votes
5
replies
5.9k
views
edgeR and Normalization of RNAseq data using ERCC controls
edger
ercc
10.5 years ago
John
▴ 10
9
votes
4
replies
3.3k
views
News:
Experimental data package 'seqc'
seqc
rsubread
featurecounts
subjunc
ercc
News
10.5 years ago
Wei Shi
★ 3.6k
6 results • Page
1 of 1
Recent ...
Replies
Comment: Check removeBatchEffect effectiveness
by
Gordon Smyth
52k
Both of your matrices, `design_batcheffects` and `design_timepoint`, span the intercept term, which will cause a warning about NA coefficie…
Comment: DiffBind - question on the use of filtered or unfiltered input data
by
sloperun3az
• 0
Your essay was beneficial to me, and I found it to be very useful. Thank you for writing it. You give a wealth of knowledge that is not onl…
Comment: compute_fasta_digest does not match Salmon index hash
by
sloperun3az
• 0
The people who are reading your work are not taken into consideration, even though you appear to have a solid understanding of the subject …
Comment: parameters of filterByExpr
by
Paul Harrison
▴ 110
It might be worth trying limma-voom (or limma-trend) instead of a GLM based method. In our experience these are less prone to this problem.…
Comment: limma::voom vs edgeR::voomLmFit - when to use?
by
Gordon Smyth
52k
voomLmFit() is in edgeR instead of limma because it needs to call edgeR::glmFit() internally to determine which of the zero observations al…
Votes
Comment: Check removeBatchEffect effectiveness
Answer: Check removeBatchEffect effectiveness
A: what should I do with many zero counts from Salmon quantification
Comment: parameters of filterByExpr
GAlignments Cigar to GRanges
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Gordon Smyth
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caroline.zanchi
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