Bioconductor WGBS

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DSS WGBS coverage

updated 8 months ago by j.gilbert • 0 • written 7.3 years ago by floriandeckert ▴ 30

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rnbeads wgbs

7.4 years ago Theo ▴ 10

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bsseq WGBS

8.2 years ago Welliton de Souza ▴ 70

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Answer: Selection of correct coefficients or contrasts in DESeq2 by swbarnes2 ★ 1.4k

I think you need to include the design you used, and the command you used to call it. The simplest answer is that your two ways of doing t…

Comment: Csaw normalization strategies - general inquiry by Luca • 0

Hello Aaron, About the IP efficiencies, yes definitely and for us, to complicate this experiment further, these batches were generated by …

Comment: How to compare expression levels of genes between scRNAseq and bulkRNAseq? by Quang NN ▴ 10

Thank you ATpoint for your response. In bulkRNAseq, I did have two cell types to compare. But, I want to also see if bulkRNAseq might have …

Answer: Why select top highly expressed genes based on normalized counts, but then plot by ATpoint ★ 4.6k

The choice of variable genes is typically part of a QC process. You select genes that in an unbiased fashion (unbiased because variance cal…

Comment: How to compare expression levels of genes between scRNAseq and bulkRNAseq? by ATpoint ★ 4.6k

My take is that RNA-seq (be it bulk or single-cell) is inherently relative. I don't think you can really "compare" the absolute expression …

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A: DESeq2 interaction term

Comment: Recount3 data correspond to TCGA data

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Scholar to Aaron Lun ★ 28k