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Comment: More regions in union when increasing DBA$config$mergeOverlap by Ian D. ▴ 70

Hello. Thanks for the reply. Sorry about this. My testing is telling me that 1 is the default (a gap), not -1 (1bp overlap). Negative …

Comment: Differences in differential analysis methods for RNA-Seq by ATpoint ★ 4.1k

> I am just re-processing due to being uncertain about how they used the edgeR analysis to reach gene level edgeR devs published something…

Comment: Differences in differential analysis methods for RNA-Seq by james.zhang20 • 0

Thanks for the explanations, it has helped clarify some things. Unfortunately, I am working from an early access public dataset (https://do…

Answer: Deseq2 and Normalization of RNA sequencing data by ATpoint ★ 4.1k

There is no expectation of normality that is realistic in terms of expression level. Levels are widely different between genes, and counts …

Comment: Differences in differential analysis methods for RNA-Seq by ATpoint ★ 4.1k

MAGeCK is a command line tool that is easy to use. I recommend you use it because a) the logFCs from DESeq2 and MAGeCK will be very similar…

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A: Error in DESeqDataSet : some values in assay are not integers

Bioconductor 3.19 is Released!

Answer: DEseq2 coefficient

SPIA plotP giving error

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Popular Question to rohitsatyam102 ▴ 20