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petra.lenzini
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Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper
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k.roelfs
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For tissue T1 and each time point, each replicate corresponds to a single plant.
For tissue T2, we actually had to sample 5 plants per rep…
Answer: edgeR: Usage of estimated dispersion from replicates for pools of the same exper
by
Gordon Smyth
47k
> Due to the high degree of heterozygosity of the plants used for the experiment, we thought it best to pool the same tissue under the same…
Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper
by
Gordon Smyth
47k
How many plants are used for each of the biological replicate samples? One plant or a few?
Comment: edgeR: Usage of estimated dispersion from replicates for pools of the same exper
by
k.roelfs
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0
Yes you are right, we have 24 RNA-seq libraries in total.
The replicates come from the same population of plants, but independent from tho…
Comment: cell_count column in SpatialExperiment Object
by
alan.ocallaghan
▴
120
Of the columns there, sum and total both refer to the total number of transcripts per observation (per spot, presumably, for Visium data). …
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